Isolation of GFP^hi and GFP^lo cells from CD41-GFP transgenic zebrafish by flow cytometry. (A) The left panel shows the distribution of CD41-GFP⁺ cells in single-cell suspension of WKM derived from Tg(CD41:GFP) zebrafish. The frames outline the gatings used to define the CD41-GFP^lo and CD41-GFP^hi subsets. Viable cells were selected based on propidium iodide exclusion. FSC-A indicates forward scatter; and SSC-H, side scatter. The middle panels locate the CD41-GFP^lo and CD41-GFP^hi cells on scatter plots of viable cells derived from WKM. FSC-H indicates forward scatter; and GFP-A, GFP-positive. The right panels are fluorescent micrographs at 10× and 60× (inset) of flow-sorted CD41-GFP^lo and CD41-GFP^hi cells. (B) The direct visualization of CD41-GFP^lo and CD41-GFP^hi cells in the kidney of Tg(CD41-GFP) fish at 10× (scale bar = 50 μm) and 60× magnification (scale bar = 10 μm).