Effect of systemic BIO administration compared with vehicle (DMSO) on murine proximal tibial bone parameters as measured by μCT. (A) Mean volume of the ROIs analyzed to confirm comparability between groups. (B) The 3-dimensional μCT rendering (right) of a control proximal tibia with ROI (left). The 3-dimensional reconstruction of ROIs from binary images (right). (C) Mean Tb N. (D) Mean Tb Sp. (E) Relative serum ALP activity. (F) Mean percentage of BV/TV. Statistics analyzed by Student t test for n = 10 mice per group; experiment was performed twice.