Pathology and biochemical markers in tumor-bearing mice treated with a peritumoral dose of BIO or vehicle (DMSO). (A) Serum PYD and TRAP 5b in response to peritumoral BIO administration. Despite values for P > .05, BIO-treated mice have generally lower levels of circulating resorption markers. (B) ELISA detection of soluble β-catenin in tissue lysates generated from the injection sites of treated and untreated animals. BIO treatment increases β-catenin levels, *P < .05. (C) Pathologic scoring on a 5-point linear scale of bone-tumor interface. Tumor necrosis and tumor cells refer to the sectional area containing dead or live tumor cells, respectively. Error bar represents SEM for n = 4-5 samples, *P < .05. Experiment was performed twice.