Quantification of HNP and proHNP in HNP-1 mice with or without serglycin. Western blotting of neutrophils isolated by density separation of murine BM cells from wild type mice, HNP-1 mice (+HNP-1), and HNP-1 mice deficient in serglycin (+HNP-1, SG^−/−) compared with human polymorphonuclear neutrophils (hsPMN) diluted to a concentration 1/50 of the murine BM cells. Detection was done with Abs against HNP, proHNP, and GAPDH.