Localization of serglycin in relation to HNP-1 in granulocytic BM cells of transgenic HNP-1 mice. Murine BM from transgenic HNP-1 mice was extracted and nongranulocytic cells diminished by ammonium chloride-based lysis and immunomagnetic sorting. Cells were fixed with paraformaldehyde and spun onto slides. (A) May-Grünwald staining of sorted cells: Overview (left) and 2 close-ups. Bars represent 20 μm (left) or 5 μm. (B-C) Immunofluoresence microscopy: Primary Abs were detected with Alexa Fluor 594 or 488 Abs and DNA was stained with DAPI. Red bars represent 5 μm. (B) Cells IgG control (red and green). (C) Cells stained for serglycin (red) and HNP-1 (green). Images were acquired with a Zeiss LSM 700 microscope and merged with Zen 2009 LE software.