Effects of the inhibition of osteoclastic bone resorption on hematopoiesis. (A) ALN-treated mice have increased HPC frequency in the BM as measured by the CFU-C assay. (B) The total number of the HPC per tibia remains unchanged. Data are mean ± SEM; n = 12. The proportion (C) and the actual number (D) of the HSCs in the BM of ALN-treated mice are reduced compared with the untreated age-matched controls as measured by the LTC-IC assay and the immunophenotyping enumeration by FACS analysis (E-F). (G) The proportion of lineage-negative cells found in the different phases of the cell cycle is changed in the ALN-treated animals compared with control; n = 3. (H) Representative graphs from 3 different experiments. *P < .05. **P < .01. ***P < .001. ns indicates not significant.