Effects of SHP-2 knock-down in KCL22-R. Effects of 5μM IMA treatment in KCL22-R^SHP2− and KCL22-R^Sh−Control on cell viability assessed by trypan blue exclusion (A), on apoptosis induction assessed by Annexin V/PI test (B), and on cell proliferation assessed by MTS assay (C). Results are expressed as mean ± SD of 3 independent experiments. (D) Signaling pathway analysis in KCL22-R^Sh−Control (referred as KCL22-R^C in the figure) and KCL22-R^SHP2− cell lines after exposure to 1μM IMA for 30 minutes. Cell extracts are subjected to WB analysis with the use of the specified antibodies.