SNP inhibits collagen-induced platelet aggregation and secretion in wild-type and sGC deficient platelets. (A-B) Washed platelets from wild-type (sGC^+/+; A) or sGC β₁ deficient mice (sGC^–/–; B) were incubated with selected concentrations of SNP for 10 minutes, and collagen (1 μg/mL) was added to induce aggregation and secretion. (C-D) Statistical data of aggregation (C) and secretion (D) shown are from 4 independent experiments. Values were normalized with respect to wild type platelets stimulated with collagen in the absence of SNP. Statistical significance between platelets in the absence of SNP and platelets preincubated with a certain concentration of SNP was determined using a Student t test.