Proportions of cytokine producing CD4+ T cells after SEB stimulation. (A) The TH subsets defined by their cytokine secretion profile after SEB stimulation are expressed as the percentage of the total CD4+ T cells; TH1 (IL-2+IFN−γ+IL-4−), TH2 (IL-2+IFN-γ−IL-4+), TH17 (IL-17+), or THpp (IL-2+IFN-γ−IL-4−). (B) The TH subsets defined by their cytokine profile as above, TH1, TH2, TH17, and THpp, after stimulation with soluble anti-CD3 and anti-CD28 mAbs. (C) Proportions of CD4+ T cells producing either 3 or 4 cytokines simultaneously after stimulation with SEB, polyfunctional T cells 3 (PFT 3; IL-2+IFN-γ+TNF-α+) or PFT 4 (IL-2+IFN-γ+TNF-α+MIP-1β+), respectively. For all plots, triangles represent values for individual samples, and the rectangles represent averages for each tissue type expressed as a percentage of total CD4+ T cells. *P < .05, **P < .01, and ***P < .001 compared with FL.