Cell expansion of SBDS-deficient cells during erythroid differentiation. (A) SBDS-knockdown and control CD34+ HSC/Ps were induced to undergo erythroid differentiation by a combination of SCF and erythropoietin and cell counts were monitored daily by trypan blue exclusion. (B) SBDS-knockdown K562/shSBDS-3 cells were transduced with a pLNCX retrovirus expressing YPet and SBDS open reading frame (which is not targeted by the shRNA expressed). YPet-positive cells were sorted and plated at a concentration of 2 × 105 cells/mL and treated with 25 μm hemin for 5 days. Cell expansion was assessed by trypan blue exclusion. Data are the mean ± SE of 3 independent experiments. *P < .05, statistically significant results using 1-way ANOVA and Tukey test for multiple means against both S-3 + YPET and S-3 cells.
