Figure 7
Prothrombin activation on the surface of collagen-activated platelets. Isolated platelets (3 × 108/mL) were incubated with 1.4μM human prothrombin. Platelet activation and prothrombinase assembly and function were simultaneously initiated by addition of 0.1 mg/mL collagen and 5nM factor Xa. Aliquots were removed at timed intervals and analyzed by SDS-PAGE followed by Western blotting using a polyclonal antibody against prethrombin-1, which recognizes prothrombin (II), prethrombin-1 (P1), fragment 1.2.A, fragment 1.2 (F1.2), prethrombin-2 (P2), the B chain, fragment 2.A (F2.A), and fragment 2 (F2). The panel is labeled as described in Figure 2. The vertical line was inserted to indicate samples run on 2 separate gels.
