IL-21R KO T cells are decreased in MLNs after BMT. (A) Lethally irradiated BALB/c mice were transplanted with B6 BM-TCD and WT or IL-21R KO T cells (1 × 106). Spleens and MLNs were harvested 1 week after BMT and stained for FACS analysis of donor CD4 and CD8 T cells. Shown are combined data from 4 independent experiments with 20 total recipients per group. (B) CD45.1 B6 and IL-21R KO (CD45.2) T cells were cotransferred into lethally irradiated BALB/c recipients. Spleens and MLNs were harvested 4 hours after transfer and stained for FACS analysis of donor T cells. The ratio of spleen to MLN donor T cells was calculated, and connecting lines indicate WT (CD45.1) and KO (CD45.2) donor T cells isolated from individual recipients. Shown is 1 of 3 independent experiments. (C) Freshly isolated MLNs were harvested from untransplanted WT or CD45.1 B6 and IL-21R KO B6 mice and stained for FACS analysis of T-cell CCR7. Shown are combined results of 4 independent experiments with a total of 14 mice per group. *P < .05 and ***P < .001 for WT versus KO T cells.
