Figure 1
Expression of OPN during GVHD. Lethally irradiated B6/SJL recipient mice (CD45.1+) were injected with C3H.SW CD8+ T cells (CD45.2+, 2 × 106) plus C3H.SW TCD BM (5 × 106) or C3H.SW TCD BM (5 × 106) alone. (A) Levels of serum OPN in recipients after transplantation were measured by ELISA. (B) Expression of OPN in spleen, liver, and intestine from GVHD recipients was measured by quantitative real-time PCR. Values for relative fold expression are normalized to gene expression in mice at day 0. Data are mean ± SEM of 6 mice analyzed. These results were representative of 3 independent experiments. *P < .05.
