Anti-OPN treatment reduces persistence of donor T cells in recipient tissues. At day 7 and 14 after transplantation, splenic, hepatic, and intestinal intraepithelial lymphocytes were isolated from GVHD recipient mice treated with anti-OPN Ab or control IgG. Absolute numbers and percentages of donor-derived CD8⁺ T cells (CD45.2⁺CD8⁺) in these tissues were determined. Data are mean ± SEM of 6 mice for each group. These results were representative of 3 independent experiments. *P < .05.