NR4A3 regulates the clonogenic potential of hematopoietic progenitors from patients and controls. (A-B) Overexpression of NR4A3 in CD34⁺ FPD/AML cells. CD34⁺ cells were transduced with an empty lentivirus (control) and a lentivirus expressing NR4A3 (NR4A3), and GFP⁺ cells were sorted at day 4 of culture. (A) NR4A3 expression was performed by Q-PCR and normalized to RPL0 expression. (B) B/NK/myeloid clonogenic potential of CD34⁺GFP⁺ cells. The histograms represent number of positive wells in 96-plated wells. Error bars represent ± SD of 1 representative experiment performed in duplicate. One representative of 2 independent experiments giving similar results is shown (P < .01). (C-D) Effect of NR4A3 knockdown expression on clonogenic progenitors. (C) Q-PCR analysis of CD34⁺GFP⁺ cells transduced with the lentivirus expressing shRNA of NR4A3 or a scramble sequence (control). The expression of NR4A3 was normalized to HPRT expression. Error bars represent ± SD of triplicate. (D) B/NK/myeloid clonogenic potential of CD34⁺GFP⁺ cells transduced by shNR4A3 or scramble sequence (control). The histograms represent number of positive wells in 96-plated wells. Error bars represent ± SD of 1 representative experiment performed in duplicate. One representative of 2 independent experiments giving similar results is shown (P < .05).