CD151 deletion from endothelial cells affects B16F10 adhesion. (A) Fluorescently labeled B16F10 cells were allowed to adhere to monolayers of wild-type (WT) and CD151-null (KO) MLECs. Representative photos after 90 minutes are shown. White boxes (top panels) are shown at higher magnification in the lower panels. (B) B16F10 adhesion was quantitated after 30 and 90 minutes with or without TNF-α (50 ng/mL; n = 4). *P < .05; **P < .005. Note that monolayer integrity for CD151-null and wild-type MLEC cells was essentially identical, as evidenced by (1) similar morphology, adhesion, spreading, and migration on gelatin22 ; (2) similar FITC-dextran permeability through monolayers (supplemental Figure 8C-D); and (3) similar electrical resistance, as measured using an electric cell-substrate impedance sensing device (data not shown). (C) Endothelial cell monolayers were removed, and then B16F10 was allowed to adhere for 30 minutes to ECM that had been deposited by either wild-type or CD151-null endothelial cells (n = 4). **P < .0005. Data are representative of 3 independent experiments with similar results. Error bars indicate means ± SEM.
