CD151 deletion from endothelial cells affects B16F10 transendothelial migration. (A) Fluorescently labeled B16F10 melanoma cells migrated (12 hours) through wild-type (WT) and knockout (KO) endothelial cell monolayers. Representative photos (2 for each condition) are shown. All fluorescent cells shown had already transmigrated and emerged through the porous filter. Some of these cells, indicated by arrows, had also undergone substantial spreading. (B) After transendothelial migration (for 3 or 12 hours), labeled B16F10 cells were counted using a fluorescent microscope (n = 4). *P < .05. (C) After the addition of B16F10 melanoma cells, dextran-FITC permeability through MLEC monolayers was determined (n = 4). **P < .005. Data are representative of 3 independent experiments. Error bars indicate means ± SEM.