SMAD3 deficiency leads to systemic accumulation of granulocytes in AHCT recipients. (A) Representative images of day 40 BM at 2 magnifications (original magnification ×2.5, 0.25-mm scale unit; and original magnification ×40, 20-μm scale unit). Virtual slides were scanned with the NanoZoomer, Version 2.0 series system and acquisition software NDP.scan Version 2.2.17 (Hamamatsu Photonics) using a 40× objective lens. (B) Representative contour plots (spleen) and (C-E) overall percentages (mean and SEM) of granulocytes (CD11b⁺/Gr-1⁺) in the spleen and mesenteric lymph nodes (C) on day 40 (n = 12 and 5, respectively) and (D) on day 20 (n = 8 and 5, respectively) after AHCT. (E) Mean percentage of granulocytes (CD11b⁺/Gr-1⁺) in the colon on day 20 after AHCT (n = 6). Statistical analyses were performed with Student t test: *P < .05.