TSG-6 inhibited nuclear translocation of NF-κB. Murine macrophages were incubated with zymosan with or without TSG-6. (A) Typical micrographs of immunocytochemistry are shown for cytoplasmic and nuclear distribution on NF-κB. (B) Quantification of data of micrographs from experiment in panel A. Values are mean ± SD for 3 random fields with at least 70 cells per field scored for each sample (n = 3; *P < .05; ***P < .0005).