Cpa3-Cre; Mcl-1^fl/fl mice have reduced numbers of basophils but not other leukocytes. (A-F) Percentages (A-C) or absolute numbers (D-F) of leukocytes (A-E) or platelets and erythrocytes (F) are depicted as values from individual mice, with bars indicating means ± SEM. *P < .05 and ***P < .001 versus corresponding values for Cpa3-Cre; Mcl-1^+/+ mice (controls). All other comparisons are not significant (P > .05). (A) Left: Percentage of basophils (FcϵRIα⁺; DX5⁺; c-Kit⁻) in the live-cell population isolated from Cpa3-Cre mouse spleen (Mcl-1^+/+, n = 6; Mcl-1^fl/fl, n = 6), BM (Mcl-1^+/+, n = 9; Mcl-1^fl/fl, n = 8), or blood (Mcl-1^+/+, n = 8; Mcl-1^fl/fl, n = 8), as analyzed by flow cytometry. Right: Representative flow cytometry plots show comparable expression of FcϵRIα⁺ on basophils isolated from Cpa3-Cre; Mcl-1^+/+ or Cpa3-Cre; Mcl-1^fl/fl mouse blood. (B) Percentage of neutrophils (Gr-1⁺) in the live-cell population isolated from Cpa3-Cre mouse spleen, BM, or peritoneal lavage fluid (Mcl-1^+/+, n = 11; Mcl-1^fl/fl, n = 10), as analyzed by flow cytometry. (C) Percentage of T cells (CD3⁺) in the live-cell population isolated from Cpa3-Cre mouse spleen (Mcl-1^+/+, n = 8; Mcl-1^fl/fl, n = 7) or thymus (Mcl-1^+/+, n = 6; Mcl-1^fl/fl, n = 6), as analyzed by flow cytometry. (D) Numbers of lymphocytes and neutrophils in blood from Cpa3-Cre mice (Mcl-1^+/+, n = 11; Mcl-1^fl/fl, n = 10). (E) Numbers of monocytes (Mcl-1^+/+, n = 8; Mcl-1^fl/fl, n = 8) and eosinophils (Mcl-1^+/+, n = 4; Mcl-1^fl/fl, n = 7) in blood from Cpa3-Cre mice. (F) Numbers of platelets and erythrocytes in blood from Cpa3-Cre mice (Mcl-1^+/+, n = 11; Mcl-1^fl/fl, n = 10).