KLF10 expression in response to hindlimb ischemia in mice and in human patients with PAD. (A) Eight- to 10-week-old, male WT mice underwent femoral artery ligation to induce hindlimb ischemia (ischemia) or sham control operation (sham), and expression of Klf10 was determined by intracellular staining of circulating PACs (Sca1⁺/VEGFR2⁺) after 7 days by flow cytometry. *P < .01 versus sham. (B) Expression of KLF10 was determined in circulating PACs (CD34⁺/VEGFR2⁺) obtained from healthy control subjects or patients with symptomatic PAD. *P < .01 versus healthy controls.