Dimorphic effects of ADAM10 on granulopoiesis. (A) Serum G-CSF levels in control (Ctrl) and Adam10/Mx1 (A10/Mx1) mice evaluated by ELISA. Bars indicate mean ± SD (n = 5). (B) Spleen/body weight ratios and total cell counts in the spleen of control and Adam10/Mx1 mice in either Gcsf^+/+ or Gcsfr^−/− genetic background. Bars indicate mean ± SD (n = 4). (C) Numbers of CD3e⁺ cells and Gr-1⁺ CD11b⁺ splenocytes in Gcsfr^−/− and Adam10/Mx1-Gcsfr^−/− mice. Bars indicate mean ± SD (n = 5). (D) Western blot analysis of ADAM10 (A10) and β-actin (βA) in BM cells from control (Ctrl) recipient mice reconstituted with Adam10/Mx1 (A10/Mx1) BM cells and Adam10/Mx1 recipient mice reconstituted with control BM cells. (E) Serum G-CSF levels in control (Ctrl) recipient mice reconstituted with Adam10/Mx1 (A10/Mx1) BM cells and Adam10/Mx1 recipient mice reconstituted with control BM cells. (F) Spleen/body weight ratios and total cell counts in the spleen of control recipient mice reconstituted with control BM cells, control recipient mice reconstituted with Adam10/Mx1 BM cells, and Adam10/Mx1 recipient mice reconstituted with control BM cells. Bars indicate mean ± SD (n = 10). Flow cytometric analysis (G) and total cell counts (H) of Gr-1⁺ CD11b⁺ cells and CD3e⁺ cells in the spleen of control recipient mice reconstituted with control BM cells, control recipient mice reconstituted with Adam10/Mx1 BM cells, and Adam10/Mx1 recipient mice reconstituted with control BM cells. Note that there was also a decrease in a T-cell population (CD3e⁺ cells) in the spleen of control recipient mice reconstituted with Adam10/Mx1 cells (H), consistent with previous findings that the effect of Notch signaling in T-cell development is cell autonomous.²³  Bars indicate mean ± SD (n = 10). The flow cytometric analysis shown here is a representative result of 3 independent experiments. t test: *P < .05, **P < .005.