Msr1 deletion does not affect the function of normal HSCs. (A) Bone marrow cells from WT and Msr1^−/− mice were analyzed by FACS for the percentages of total HSCs (Lin⁻c-Kit⁺Sca-1⁺), LT-HSCs (Lin⁻c-Kit⁺Sca-1⁺CD34⁻), ST-HSCs (Lin⁻c-Kit⁺Sca-1⁺CD34⁺Flt3⁻) and MPPs (Lin⁻c-Kit⁺Sca-1⁺CD34⁺Flt3⁺). (B) Cells from bone marrow and peripheral blood of WT and Msr1^−/− mice were analyzed by FACS for the percentages of Gr-1⁺, B220⁺ and CD3E⁺cells. (C) 3 doses (1 × 10⁵, 5 × 10⁴ and 2.5 × 10⁴) of WT or Msr1^−/− bone marrow cells were injected into lethally irradiated recipient mice. Survival curves showed that there was only a minor engraftment defect of bone marrow cells in Msr1^−/− mice. (D) Msr1^−/− (CD45.2) and WT (CD45.1) bone marrow cells were 1:1 mixed and then transferred into recipient mice (n = 5). 12 weeks after transplantation, FACS analysis showed the percentages of WT and Msr1^−/− cells in peripheral blood of recipient mice.