Reduced CD1a and pSTAT5 in MoDCs from viremic patients who had received a transplant. Monocytes were isolated from the blood of patients who had received a transplant with the use of Ficoll gradients followed by sorting with anti-CD14 magnetic separation beads. Cells were differentiated into MoDCs in culture medium containing GM-CSF and IL-13 for 5 days. (A) Light microscopy of monocytes after 3 days in culture (×200; Zeiss, Telaval 31). Images were acquired with a Nikon Eclipse TE 2000_v; 20×/0.35 objective; culture medium 37°C; camera: Nikon DX71200C; Adobe Photo-shop 9.02. (B) The percentage of MoDCs expressing CD1a was determined by flow cytometry. (C-D) Amounts of IL-6 secreted by MoDCs (C) and in plasma of patients who received a transplant (D) were determined by CBA. (E) Total and phosphorylated STAT5 (pSTAT5) was assessed by Western blotting of MoDC extracts with β-actin as control. The histogram below the Western blots represents quantification of proteins from all the patients. Lines represent median values. Mann-Whitney U tests were used to determine statistical significance.