American Society of Hematology

Figure 1

$Figure 1. Recognition of HPLC fractions of HLA-A2–eluted peptides by the allo-HLA–reactive T-cell clones. (A) The allo-HLA–reactive T-cell clones were stimulated with HLA-A2–positive EBV-LCLs (A2pos LCL), HLA-A2–negative EBV-LCLs (A2neg LCL), HLA-A2–negative EBV-LCLs transduced with HLA-A2 (A2 td), T2 cells (T2), and T2 cells loaded with a mixture of different HLA-A2–binding peptides from CMV, EBV, and Flu (T2+virpep). Supernatants were harvested after 18 hours of stimulation and IFNγ was measured by standard ELISA. (B) Peptides were eluted from the HLA-A2 molecules of EBV-LCLs and fractionated by RP-HPLC using a water/acetonitrile/TFA gradient. Twenty-six of the 44 non-T2 recognizing allo-HLA-A2–reactive T-cell clones, of which 8 are shown in this figure, were stimulated with T2 cells loaded with the HPLC fractions.$

Recognition of HPLC fractions of HLA-A2–eluted peptides by the allo-HLA–reactive T-cell clones. (A) The allo-HLA–reactive T-cell clones were stimulated with HLA-A2–positive EBV-LCLs (A2pos LCL), HLA-A2–negative EBV-LCLs (A2neg LCL), HLA-A2–negative EBV-LCLs transduced with HLA-A2 (A2 td), T2 cells (T2), and T2 cells loaded with a mixture of different HLA-A2–binding peptides from CMV, EBV, and Flu (T2+virpep). Supernatants were harvested after 18 hours of stimulation and IFNγ was measured by standard ELISA. (B) Peptides were eluted from the HLA-A2 molecules of EBV-LCLs and fractionated by RP-HPLC using a water/acetonitrile/TFA gradient. Twenty-six of the 44 non-T2 recognizing allo-HLA-A2–reactive T-cell clones, of which 8 are shown in this figure, were stimulated with T2 cells loaded with the HPLC fractions.

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