American Society of Hematology

Figure 2

$Figure 2. Investigation of the peptide specificity of T2-reactive T-cell clones. (A) T2-reactive T-cell clones HSS27, HSS29, and HSS43 were stimulated with HLA-A2–positive EBV-LCLs (A2pos LCL), HLA-A2–negative EBV-LCLs (A2neg LCL), T2 cells (T2), and Drosophila cells expressing HLA-A2, CD80, and CD54 (Dros+A2). (B) Peptides were eluted from the HLA-A2 molecules of T2 cells and fractionated by RP-HPLC. The 6 allo-HLA–reactive T-cell clones which recognized T2 cells were tested for IFNγ production against the HPLC fractionations loaded on Drosophila cells expressing HLA-A2, CD80, and CD54; 3 representative clones are shown.$

Investigation of the peptide specificity of T2-reactive T-cell clones. (A) T2-reactive T-cell clones HSS27, HSS29, and HSS43 were stimulated with HLA-A2–positive EBV-LCLs (A2pos LCL), HLA-A2–negative EBV-LCLs (A2neg LCL), T2 cells (T2), and Drosophila cells expressing HLA-A2, CD80, and CD54 (Dros+A2). (B) Peptides were eluted from the HLA-A2 molecules of T2 cells and fractionated by RP-HPLC. The 6 allo-HLA–reactive T-cell clones which recognized T2 cells were tested for IFNγ production against the HPLC fractionations loaded on Drosophila cells expressing HLA-A2, CD80, and CD54; 3 representative clones are shown.

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