CD5 induces elevated levels of IL-23R. (A) Naive T cells were stimulated via CD3/CD28 or CD3/CD5 in Th17-polarising conditions. IL-23R expression was measured by FACS at day 3, day 6, and day 11. Data shown are mean ± SEM of 9 individual experiments using different donors. (B) IL-23R expression of stimulated naive T cells described under (A) was measured by FACS. Data shown are from 1 representative experiment of 9 independent experiments using different donors. (C) Naive T cells were stimulated via plate-bound antibodies directed against CD3/CD5 in Th17 inducing conditions. IL-23R⁺ and IL-23R⁻ cells were sorted at day 4 and cultured for another 7 days. IL-17 was measured by ELISA after 24 hours of stimulation with anti-CD3/PdBu (right panel). Data shown are from one representative experiment of 3 independent experiments with different donors (n.d., not detectable). (D) Naive T cells were stimulated with CD3/CD28 or CD3/CD28 and sorted for IL-23R expression as described in (C). IL-17 was measured 7 days after sorting of anti-CD3/PdBu stimulated cells (24 hours). Data shown are from 3 individual experiments using different donors.