ETNK1 mutations in patients with aCML/chronic myelomonocytic leukemia. (A) Outline of the ETNK1 protein. The yellow region highlights the catalytic domain of the protein. The red band corresponds to the mutational hotspot. In the upper part, a conservation analysis of the mutated locus is shown. The red and blue circles highlight the position of individual mutations identified in aCML and chronic myelomonocytic leukemia, respectively. (B) Intracellular phosphoethanolamine/phosphocholine ratio in patients with aCML. The red bars show the intracellular phosphoethanolamine/phosphocholine ratio of leukemic (myeloid) cells in 3 ETNK1 mutated patients (8T, 13T, and 19T, corresponding to patients CMLPh-008, CMLPh-013, and CMLPh-019). The blue bars show the intracellular phosphoethanolamine/phosphocholine ratio of myeloid (3T, 6T) or lymphoid (3H, 6H) cells negative for ETNK1 mutations (patients CMLPh-003 and CMLPh-006). The light red and light blue bars indicate the intracellular phosphoethanolamine/phosphocholine ratio of a matched myeloid/lymphoid sample whose leukemic (myeloid) cells are positive for ETNK1 mutation. The error bars represent the standard deviation of 3 experiments. The asterisks indicate the significance level: *P < .05; **P < .005.