Association of ZBP-89 chromatin occupancy with activating gene features. (A) DNA sequence motif preference at ZBP-89 bound sites across the entire ENCODE array using cis-regulatory element annotation system algorithm.²⁰  (B) Pie chart showing the relative location of ZBP-89 occupancy peaks relative to annotated gene structures. (C) Venn diagram showing overlap of genes containing ZBP-89 occupancy peaks and those with enrichment for 3meH3K4 and/or 3meH3K27 between −2 kb upstream to 2 kb downstream of the TSS. (D) Venn diagram showing overlap of ZBP-89 occupied genes, RNA Pol II-occupied genes, and genes enriched for AcH3. (E) Plot showing percentage frequency of ZBP-89, RNA Pol II, AcH3, 3meH3K4, and 3meH3K27 enrichment peaks relative to distance from the TSS. (F) Plots of normalized cDNA gene expression microarray probe intensity signals²⁹  (NCBI GEO accession no. GSE22552) corresponding to the 84 ZBP-89 bound genes (represented by 219 probes; red) compared with the mean of 10 sets of randomly selected 219 probe sets (blue). Data for each of the represented equivalent erythroid maturational stages (CFU-E), proerythroblast (Pro-E), intermediate erythroblast (Int-E), and late erythroblast (Late-E) are shown. All probe sets to the left of the vertical arrows have intensities ≥ 1.5-fold greater than the random control probe sets of equivalent rank order.