Treatment of LSCs in vitro with Δ¹²-PGJ₃ increases p53 mRNA expression. Dose response of p53 mRNA expression in LSCs sorted from FV-infected (A) or BCR-ABL⁺ HSC–transplanted spleens (B). (C) Real-time PCR analysis of the time course of p53 expression in FV-LSCs treated with Δ¹²-PGJ₃ or vehicle control. (D) Analysis of p53 mRNA expression in the spleens of FV-infected mice after treatment with Δ¹²-PGJ₃ or vehicle control. (E) Analysis of nuclear and cytoplasmic p53 protein localization in FV-LSCs treated with Δ¹²-PGJ₃ (25nM) or vehicle control. (F) Analysis of apoptosis of MEL cells treated with Δ¹²-PGJ₃ or vehicle control. All data are representative 3 experiments.