LSK cells from BM of Flt3-ITD mice have reduced serial replating capability. (A-B) Thirty sorted LSK cells from BM of WT, Flt3^+/ITD, Flt3^ITD/ITD, and Flt3^−/ITD mice were plated in methylcellulose containing stem cell factor (SCF), IL-3, and/or FL. The first and secondary replating colony-forming assays were done after 7 days of culture in the presence of the aforementioned cytokines. Results are mean (SD) values of 2 experiments (3 mice of each genotype, each performed in duplicate). *P < .05. **P < .01.