American Society of Hematology

Figure 2

$Figure 2. Representative ddPCR experiment. (A) Wild-type DNA reveals only HEX-positive droplets. (B) Positive control DNA from a patient with a SETBP1 p.G870D mutation reveals a mixed population of HEX- and FAM-positive droplets. Orange droplets indicate the presence of mutant and wild-type DNA within 1 droplet. (C) When no DNA is added, no template control wells contain only empty droplets. No HEX- or FAM-positive droplets are identified. (D) DNA from sample J435 reveals the presence of subclonal SETBP1 p.G870D mutations in 3 droplets, representing an allelic fraction of 0.09%.$

Representative ddPCR experiment. (A) Wild-type DNA reveals only HEX-positive droplets. (B) Positive control DNA from a patient with a SETBP1 p.G870D mutation reveals a mixed population of HEX- and FAM-positive droplets. Orange droplets indicate the presence of mutant and wild-type DNA within 1 droplet. (C) When no DNA is added, no template control wells contain only empty droplets. No HEX- or FAM-positive droplets are identified. (D) DNA from sample J435 reveals the presence of subclonal SETBP1 p.G870D mutations in 3 droplets, representing an allelic fraction of 0.09%.

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