TCL1 expression in CLL-like cell lines inversely correlates with expression of c-fos and phosphorylation of c-jun. (A) Whole cell extracts of CLL-like cell lines Mec1 and WaC3CD5 were subjected to immunoblot analysis using antibodies indicated in the figure. (B) Real-time RT-PCR analysis of c-fos and c-jun in total RNA extracted from Mec1 and WaC3CD5 cells. The experiment was repeated twice with triplicate measurements. Error bars represent SD. P values were calculated using the Student t test. (C) WaC3CD5 cells ectopically expressing TCL1 were generated by retroviral infection. Western blot analysis using antibodies indicated in the figure was performed on whole cells extracts from vector control and TCL1 expressing WaC3CD5 cells. Quantification of bands represented as column graph.