Wnt4 is predominantly expressed byTECs. Relative mRNA expression for Wnt4 in total TECs (CD45−EpCAM+), cTECs (Ly51+), immature mTECs (Ly51−MHCII−), mature mTECs (Ly51−MHCII+), endothelial cells (CD45−CD31+), fibroblasts (CD45−EpCAM−Ly51+), and in T-cell subsets (DN, DP, CD4 SP, and CD8 SP) of E18.5 (A) and 4-week-old adult (B) mice, expressed as mean 2−ΔCT standardized to the endogenous control GAPDH. (C) Relative mRNA expression of Fz receptors in adult TEC subsets is expressed as mean 2−ΔCT standardized to the endogenous control GAPDH. Histogram represents mean ± SEM of 3 mice.