Figure 2
Figure 2. Wnt4 is needed for steady-state postnatal thymopoiesis. (A) Thymic cellularity from E15.5 and E18.5 Wnt4+/+ and Wnt4−/− mice. (B) Thymic cellularity from mice in which Wnt4 deletion has been induced (Cre+) on E18.5 or in the adult, compared with Cre− littermate controls. (C-E) FACS analysis of lineage-negative thymocytes from E18.5 Wnt4+/+ and Wnt4−/− mice (C), mice in which Wnt4 has been deleted at birth (D), and mice in which Wnt4 has been deleted in adulthood (E). The graphs on the left show the proportion of ETP (Lin−cKithiCD25−), DN2 (Lin−cKithiCD25+), and DN3 (Lin−cKit−/loCD25+) subsets expressed as percentage of total thymocytes. The graphs in the middle show the absolute numbers of cells within each subset per thymus. Representative flow cytometry data are shown on the right. All histograms represent the mean ± SEM (n = 6-7/group; *P < .05, **P < .01, ***P < .005).

Wnt4 is needed for steady-state postnatal thymopoiesis. (A) Thymic cellularity from E15.5 and E18.5 Wnt4+/+ and Wnt4−/− mice. (B) Thymic cellularity from mice in which Wnt4 deletion has been induced (Cre+) on E18.5 or in the adult, compared with Cre− littermate controls. (C-E) FACS analysis of lineage-negative thymocytes from E18.5 Wnt4+/+ and Wnt4−/− mice (C), mice in which Wnt4 has been deleted at birth (D), and mice in which Wnt4 has been deleted in adulthood (E). The graphs on the left show the proportion of ETP (Lin−cKithiCD25−), DN2 (Lin−cKithiCD25+), and DN3 (Lin−cKit−/loCD25+) subsets expressed as percentage of total thymocytes. The graphs in the middle show the absolute numbers of cells within each subset per thymus. Representative flow cytometry data are shown on the right. All histograms represent the mean ± SEM (n = 6-7/group; *P < .05, **P < .01, ***P < .005).

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