Generation of a niche for DCs permits intrathymic DC development. (A) Sorted thymic CX3CR1⁺ ETPs, CX3CR1⁻ ETPs, and BM MP/MDP precursors were injected intrathymically into congenic (CD45.1⁺CD45.2⁺), nonmanipulated mice. The development of myeloid cells and T cells was assessed 21 days after transfer. CD45.2⁺ T-cell–committed precursors were either DP or SP for CD4 and CD8. Myeloid cells were CD4/CD8 DN and expressed CD11c (DC), or were CD11c⁻CD11b⁺ (macrophages). (B) Precursors sorted as in (A) were injected intrathymically into CD11c-DOG (CD45.1⁺CD45.2⁺) mice, in which DCs were depleted 24 hours prior to progenitor injection. Development of T cells and myeloid cells was assessed 21 days after transfer. (C) Quantification of data shown in (B). (A-B) Representative plots of 2 independent experiments and (C) pooled data of 2 independent experiments, with each point representing an individual mouse.