Role of mTORC1 and mTORC2 in the growth of BMMC progenitors. (A) Four- to 6-week-old BMMCs from WT or KI-disrupted mTOR (KI) mice were analyzed by immunoblotting with mTOR phosphoprotein-specific or protein-specific Abs and data were quantitated (right). (B) One million BM cells from WT or KI mice were cultured for 4 weeks in the presence of IL-3 or IL-3/SCF and cell numbers were determined. (C) Total numbers of peritoneal cells recovered from WT or KI mice. (D) The peritoneal cells recovered in panel C were stained with PE-KIT-, FITC-FcϵRI-, and FITC-IgE–specific Abs and analyzed by flow cytometry. (E) The percentage of peritoneal MC population, FcϵRI, and KIT⁺ population determined in panel D was evaluated. In panel D, a representative flow cytometry analysis is shown. In panels A through C and E, the data represent means and SEM (A-B, n = 3; C and E, n = 6; note that “n” refers to the number of pairs of mice) and differences between WT and KI (*P < .05 by t test) are indicated.