BCL11A occupancy in BGL3 changes when γ-globin is reactivated. ChIP was performed using a BCL11A Ab to determine occupancy in low- and high-HbF cells. Solid black bars indicate low-HbF cells; white bars, high-HbF cells. Dark and light gray bars indicate values for an isotype control Ab in low- and high-HbF cells, respectively. The dotted line indicates the average of IgG control precipitations across all amplicons. Results were calculated and plotted as described in Figure 2D. *P < .05.