Long-range interactions in the β-globin locus include the BGL3 region. 3C analysis was performed for cells expressing high- and low-HbF. Each EcoRI cleavage site is represented on the genome browser by a yellow triangle, and the interaction frequency with the designated anchor fragment is plotted in the middle of each fragment (dotted vertical lines). The globin genes and BGL3 sequences are shown in the middle track as gray and black rectangles, respectively. Colored line graphs represent data from low-HbF cells (green) and high-HbF cells (red). (A) Relative interaction frequency between the LCR2-4 fragment as anchor and other regions of the locus. (B) Relative interaction frequency between the BGL3 region and other parts of the locus. Note that the interaction frequency reported for the EcoRI fragment containing the ^Gγ-globin gene represents the value for both γ-globin gene interactions with the LCR (see “Chromosome conformation capture”). Error bars indicate SD.