A subset of tonsil pDCs express functional CCR6 and CCR10. (A) CCR6 and CCR10 expression was analyzed by flow cytometry on pDCs from healthy blood and tonsil mononuclear cells. Representative staining (left) and the percentages of CCR6⁺ and CCR10⁺ cells among CD123⁺ BDCA2⁺ pDCs in blood and tonsil are shown (right). Each point represents an independent donor (n = 10); and horizontal bars, group medians. The P value was obtained by a nonparametric Wilcoxon test. (B) Percentages of CCR6⁺ CCR10⁺ cells among BDCA2⁺ pDCs from tonsil mononuclear cells are indicated, and data are representative of 3 independent experiments. (C) pDCs were enriched from tonsils by eliminating CD2⁺ cells. Chemotactic activity of tonsil pDCs among enriched cells toward CXCL12, CCL20, and CCL28 was analyzed. Results are expressed as migration index or percentage input according to the number of C(X)CR-expressing pDCs of the input population (inset). **P < .01 (Wilcoxon test). *P < .05 (Wilcoxon test).