IL-12 treatment induces the expression of miR-132, miR-212, and miR-200a in NK cells. NK cells were treated with 0 or 10 ng/mL IL-12 and incubated for indicated times in presence of IL-2. Total small RNA were purified from the respective cell pellets and analyzed by qRT-PCR for the expression of miR-132 (A), miR-212 (B), miR-200a (C), and miR-141 (D). miRNA expression was normalized with control RNU6B. 2 + 12 / 2 represents that the fold changes in mRNA levels were calculated by comparing the value of IL-2+IL-12–cultured cells (2 + 12) to that of IL-2 cultured samples²  in parallel. The results are mean ± SEM of representative experiment with NK cells from 3 different donors. *P < .05, **P < .01 vs 0 hours untreated cells.