Representative tandem mass spectrum of a candidate palmitoyl peptide derived from TLT-1 protein. Enriched palmitoyl proteins were separated by SDS-PAGE and a gel slice containing 30- to 50-kDa proteins excised. Proteins were digested in gel, followed by the extraction of tryptic peptides, which were analyzed by LC-MS/MS. Free cysteines in the purified peptides are candidate palmitoylation sites.