HEV pockets are plastic structures. (A) The right panel in the diagram represents a situation in which lymphocytes are not engulfed within pockets but simply migrating between 2 ECs while the left panel represents lymphocytes present in a true structured pocket underneath the HEV. L = Lymphocyte. (B) Confocal image of a LN section from a ubiquitin-GFP chimera (green) stained for ICAM-1 (red) and sytox 63/nuclei (blue) Arrowheads indicate the inner membrane of the EC directly in contact with lymphocytes. (C) Confocal image of a LN section from a ubiquitin-GFP chimera (green) stained for ERTR-7 (red), Desmin (white) and sytox 63/nuclei (blue). Arrows indicate the membranes of myofibroblasts/pericytes that ensheath the conduit system. Data are representative of 3 different experiments (2 mice per experiment).