Primary Treg transcriptome study. (A) Gene ontology analysis of regulated gene transcripts in GVHD. Analysis was performed using the GeneSpring GX11 software on the 3159 transcripts with a fold change ≥ 2 or ≤ −2 for at least 1 of the predefined 6 comparisons (“Data analysis”) and a P value < .1. (B) Venn diagram analysis of the Treg transcriptomes based on the 3159 transcripts with a fold change ≥ 2 or ≤ −2 for at least 1 of the 6 comparisons (“Data analysis”). The differential analysis of the Treg transcriptomes from patients at day 90 and day 150, respectively, with and without developing a GVHD after SCT revealed, n = 1233 and n = 429, respectively, regulated gene transcripts. Independent of the analyzed time point, the differential Treg transcriptome analysis of patients with and without developing a GVHD identified n = 161 regulated gene transcripts with a fold change ≥ 2 or ≤ −2.