Regulated miRNAs in Tregs of SCT patients. Patients without GVHD and with GVHD manifestation before day 100 and after day 100 after SCT are summarized as “no GVHD” or “GVHD” > 100 days and > 100 days, respectively. CD4⁺CD25^hiCD127^lo/− Tregs were sorted from PBMCs of blood withdrawals with a purity > 95%. Isolated total RNA from n = 3 patients without GVHD and n = 3 patients with GVHD < 100 days and > 100 days were pooled, respectively. In addition, total RNAs were pooled from n = 3 healthy donors (HD). Results are presented as a heat map generated using Genesis software (Institute for Genomics and Bioinformatics, Graz University of Technology, Austria) and show 163 differentially regulated miRNAs with a log median fold change > 0.4 or < −0.5 in GVHD vs no GVHD < 100 days and/or GVHD vs no GVHD > 100 days. Red frames highlight miRNAs with predicted targets of gene transcripts encoding GZMA, LGALS1, CCR5, CD44, CXCR3, and CCR1.