miR-135b blockade suppresses IL-17 production by ALCL cells and modulates ALCL immunophenotype. (A) Regulation of Th17-related molecules by miR-135b. Karpas 299 cells were infected with lentivirus harboring TuD-NC or TuD-miR-135b and applied to qRT-PCR analysis. Effects of miR-135b inhibition on the transcripts of Th17-related molecules were analyzed by qRT-PCR assay. Effects of miR-135b inhibition on IL-6 and IL-8 expression, as determined in panel A. (C) Effects of miR-135b inhibition on IL-17F production were analyzed in Karpas 299 cells by ELISA. (D) Effects of miR-135b inhibition on granzyme B (GZMB) and perforin 1 (PRF1) expression, as determined in panel A. miR-135b blockade did not affect the expression levels of IL-21, IL-23, and TGF-β1 (data not shown; *P < .05; **P < .01; ***P < .001).