Regulation of paracrine inflammatory reaction and tumorigenic potential by miR-135b. (A) ALCL cells stimulate proinflammatory cytokine (IL-1β, IL-6, and IL-8) and chemokine (CCL2, CCL7, CCL20, CXCL1, and CXCL2) production in human fibroblasts through an miR-135b–dependent manner. WI-38 human fibroblasts were cocultured with Karpas 299 or Jurkat cells and subjected to qRT-PCR assay (*P < .05; **P < .01). (B) Growth curves of Karpas 299 subcutaneous tumors after transplantation into SCID mice and administration with miR-135 antisense–atelocollagen or control oligonucleotide–atelocollagen complexes. After random assignment at day 7 after inoculation (n = 6/group), LNA-based antisense oligonucleotides (5μM) with atelocollagen were administered into the subcutaneous spaces around the tumors at days 7 and 10 and measured (means ± SEM; ***P < .001). (C) Effects of miR-135b inhibition on tumor angiogenesis. Representative images of CD31 immunostaining of tumor sections (left) and quantification of microvessel density measured by CD31-positive area (right). Pixel density was quantified in multiple tumor images from 6 mice per group using ImageJ 1.36b software. Scale bar represents 100 μm (*P < .05).