Procedure for preparing the hemi-hind limb ischemic mouse model. On the right leg, we ligated the proximal end of the superficial femoral artery and vein, the origins of the popliteal artery and vein, and the distal portions of the saphenous artery and vein with surgical silk. All vessels surrounding the 3 ligated points were excised. The left leg was not treated, except for an incision in the skin of the thigh.