Neutrophils make prolonged associations with donor-derived graft-resident. (A) Intravital 2P imaging of the distribution of donor DCs (green) and recipient neutrophils (blue) within lung grafts 1 hour after B10.BR CD11c-EYFP → B6 LysM-GFP transplantation. Blood vessels appear red (nontargeted 655-nm quantum dots). Scale bar indicates 60 μm. (B) Zoomed views show several neutrophils (yellow) making contact with CD11c⁺ DCs (white). Scale bar indicates 15 μm. (C) Measurement of neutrophil contact time with DCs over a 43.5-minute period approximately 1 hour after engraftment. The bar represents the mean neutrophil-DC contact time of 6.8 minutes. Some cells remained associated for the entire imaging period.