Phenotype of monocyte/macrophage subpopulation throughout resolving inflammation. There are 2 broad categories of monocytes/macrophages, namely, Ly6c⁺F4/80⁺ and Ly6c⁻F4/80^hi revealed in Figure 5B through E. These were isolated using FACSAria at 24, 48, and 72 hours from resolving peritonitis as well as at 72 hours from peritonitis triggered by 10 mg zymosan. mRNA was extracted and quantitative PCR carried out for the relative expression of genes enriched in each population. Data are mean ± SEM for n = 3 to 6 replicates per group. Cells from these each of these replicates are pooled from 3 to 5 animals before FACS cell sort.